Bovine Thyrotropin

Novel IgA antibody to bovine thyrotropin in a healthy man.

Bovine serum hormone concentrations after thyroprotein and thyrotropin releasing hormone.

Twenty-four Holstein heifers were assigned randomly to one of four t reatment groups to be given: 1) thyroprote in (TP) for 6 days; 2) casein for 6 days; 3) TP for 27 days; or 4) casein for 27 days. Thyrotropin releasing hormone (TRH, 33.3 /~g/100 kg BW) was given at 1030 and 1330 hr on day 6 or 33 to heifers treated for 6 or 27 days, respectively. Prior to TRH treatment , serum thyroxine (T4) ...

Molecular cloning of bovine thyrotropin-releasing hormone receptor gene.

The genomic DNA encoding bovine Thyrotropin-Releasing Hormone Receptor (TRHR) was isolated from a bovine (Holstein) genomic library. Using PCR fragments of bovine candidate TRHR Transmembrane domain (TMD-III-IV) and C-terminus domain of mouse TRHR cDNA as probes, 9 x 10(5) plaques were screened to obtain several clones each containing the N-terminus or C-terminus domain. The bovine TRHR gene en...

The effects of modification of the COOH-terminal regions of bovine thyrotropin and its subunits.

Removal of a limited number of amino acid residues at the COOH termini of the peptide chains of the glycoprotein hormone, thyrotropin (TSH) shows that the integrity of these regions is important both in subunit-subunit interactions and in uivo biological response. Treatment of the native hormone with a mixture of carboxypeptidases A and B leads to loss of half of the biological activity with th...

Receptor for thyrotropin-releasing hormone in plasma membranes of bovine anterior pituitary gland. Role of lipids.

The receptor for thyrotropin-releasing hormone has been located in the plasma membranes of bovine anterior pituitary gland. Treatment of membranes with phospholipase A, phospholipase C, or digitonin or addition of lysophosphatidylcholine diminishes or completely destroys the ability of membranes to bind 3H-labeled thyrotropin-releasing hormone. Phospholipase D was without effect. These reductio...